Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Features and Benefits

Quickly desalts, removes contaminants and transfers to a new buffer in a single step. Suitable for purification of DNA from small molecules by gel filtration Larger bead size for large scale group separations where high flow rates and low operating pressures are required. Classic gel filtration medium.

General description

Sephadex^® G-50 Medium is well established gel filtration medium for desalting and buffer exchange of biomolecules >30 000 molecular weight. The larger bead size of G-50 Medium is suited for large scale separations.Sephadex^® is a gel filtration medium prepared by crosslinking dextran with epichlorohydrin. Different types of Sephadex^® differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. Sephadex^® G-50 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex^® G-50 is available in 4 different particle sizes (Course, Medium, Fine & Superfine) and Superfine has the smallest bead size for higher efficiency with shorter diffusion distances. Cource and Medium are are preferred for large scale group separations where high flow rates and low operating pressures are required.

Legal Information

Sephadex is a registered trademark of Cytiva

Storage and Stability

Store at 4 to 30 °C ()